规格：50µL/100µL
货号：PN0149
价格：1350-50 µL， 2200元/100 µL
全名 | Anti-Beclin 1 Rabbit pAb |
别名 | Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197, APG6, VPS30, Protein GT197, beclin1, beclin-1 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
别名 | Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197, APG6, VPS30, Protein GT197, beclin1, beclin-1 |
交叉反应 | Human,mouse，Rat (predicted: ,Pig, Chicken, Zebrafish, Hamster, Goat, Fish, Duck) |
产品应用 | WB： 1:1000-10000, IP： 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC-P： 1:50-500, IF-P： 1:50-500, IF-FRO： 1:50-500, IF/ICC : 1:200-1:800 |
理论/实际分子量 | 52kDa /52~60 kDa |
细胞定位 | 细胞质，细胞核 |
性 状 | Liquid |
浓 度 | 600ug/ml |
免 疫 原 | |
亚 型 | IgG |
纯化方法 | Antigen affinity purification |
Uniprot ID | |
缓 冲 液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
保存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
背景
Beclin 1, also known as ATG6 or VPS30, interacts with various cofactors (e.g. Ambra1, Barkor (Atg14), Rubicon, or UVRAG) to regulate the lipid kinase Vps34 and promote the formation of the BECLIN1-Vps34-Vps15 complex, hence inducing autophagy. Its function (via the BH3 domain) is inhibited by Bcl-2 or Bcl-XL. Beclin 1 (BECN1) is a crucial molecule in the control of the autophagic activity, and its activity is regulated by multiple mechanisms, including the post-translational modification, protein-protein interaction, and subcellular localization. It plays a role in crosstalk between apoptosis and autophagy. It has been reported that Beclin 1 can be cleaved into fragments of 50, 37 and 35 kDa during apoptosis. It is involved in many disorders, including neurodegeneration and cancer (tumorigenesis). Beclin 1 is a mammalian tumor suppressor, and its gene is monoallelically deleted in 75% of ovarian, 50% of breast, and 40% of prostate cancers. Decreased expression of Beclin 1 has also been observed in human brain and lung tumors. The level of Beclin 1 was decreased in the affected brain regions of patients with Alzheimer's disease early in the disease process. Recent studies have also shown that gain and loss of Beclin 1 function affects the death of heart cells.
