其他产品
其他产品

Caspase-3 Antibody

规格:50μl, 100μl

价格:¥1250/50μl, ¥2500/100μl

产品特性

数据表

UniProtP42574
Mol Weight32kDa
Alternative NamesCASP3; Caspase-3; APOPAIN; CPP32; CPP32B; SCA-1; Caspase3;
ImmunogenA synthesized peptide derived from human Caspase-3
StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
PurificationAffinity-chromatography
IsotypeRabbit IgG
ClonalityMonoclonal
DescriptionCaspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position.


Applications

ReactivityHuman
ApplicationWB,IHC,ICC/IF,IP,FC
Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
Western blot analysis of Caspase-3 in HEK293 cell lysate.
Immunohistochemical analysis of paraffin-embedded human tonsil, using Caspase-3 Antibody.
Immunofluorescent analysis of Hela cells, using Caspase-3 Antibody .
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.