规格：50μl, 100μl
价格：¥1400/50μl, ¥2500/100μl
数据表
| UniProt | P11021 |
| Mol Weight | 78kDa |
| Alternative Names | GRP-78; GRP78; BIP; MIF2; HSPA5 |
| Immunogen | A synthesized peptide derived from human GRP78 BiP |
| Storage | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Purification | Affinity-chromatography |
| Isotype | Rabbit IgG |
| Clonality | Monoclonal |
| Description | When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). |
Applications
| Reactivity | Human,Mouse,Rat |
| Application | WB,IHC,ICC/IF |
| Recommended dilution | WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 |
 | Western blot analysis of GRP78 BiP expression in (1) LnCaP cell lysate;(2) HepG2 cell lysate. |
 | Immunohistochemical analysis of paraffin-embedded human pancreas, using GRP78 BiP Antibody. |
 | Immunofluorescent analysis of HepG2 cells, using GRP78 BiP Antibody . |
 | All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. |
 | Immunohistochemical analysis of paraffin-embedded Rat testis, using the Antibody at 1:200 dilution. |
 | Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin's lymphoma, using the Antibody at 1:100 dilution. |
 | Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:100 dilution. |
 | Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:100 dilution. |
 | Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:200 dilution. |
 | Immunofluorescent analysis using the Antibody at 1:50 dilution. |
